Chemical studies on ribosomal ribonucleic acid and ribosome formation in HeLa cells
نویسندگان
چکیده
منابع مشابه
Studies on the Formation of Transfer Ribonucleic Acid-Ribosome Complexes I. THE EFFECT OF STREPTOMYCIN AND RIBOSOMAL DISSOCIATION ON “C-AiCIINOACYL TRANSFER RIBONUCLEIC ACID BINDING TO RIBOSOMES*
Streptomycin-sensitive 70s ribosomes which are dissociated into SOS and 30s particles by dialysis against 1 X lop4 M magnesium acetate, and then reassociated in 0.02 M magnesium acetate, differ from nondissociated 70s ribosomes in r4C-aminoacyl soluble ribonucleic acid (sRNA) binding. 1. Reassociated ribosomes bind about twice as much 14C!phenylalanyl-sRNA in the presence of polyuridylic acid a...
متن کاملStudies on the formation of transfer ribonucleic acid-ribosome complexes. I. The effect of streptomycin and ribosomal dissociation on 14-C-aminoacyl transfer ribonucleic acid binding to ribosomes.
Streptomycin-sensitive 70s ribosomes which are dissociated into SOS and 30s particles by dialysis against 1 X lop4 M magnesium acetate, and then reassociated in 0.02 M magnesium acetate, differ from nondissociated 70s ribosomes in r4C-aminoacyl soluble ribonucleic acid (sRNA) binding. 1. Reassociated ribosomes bind about twice as much 14C!phenylalanyl-sRNA in the presence of polyuridylic acid a...
متن کاملRibosome development and the methylation of ribosomal ribonucleic acid.
Immature ribonucleoprotein particles accumulate in amino acid-starved cells of a relaxed mutant of Escherichia coli. The ribonucleic acid (RNA) of these particles is nonmethylated in cells starved for methionine. However, in bacteria starved for arginine, lysine, or histidine, the RNA of these particles is one-half methylated. The relationship of submethylation to a structural alteration in the...
متن کاملThe Effect of Actinomycin on Ribosome Formation in Hela Cells.
Although the weight of experimental evidence is decidedly in favor of the view that ribonucleic acid (RNA) in animal cells is made in the nucleus and transported to the cytoplasm,'-' Harris and collaborators7-' have pointed out that no direct experiment demonstrating such a transfer has been performed. When the action of actinomycin in suppressing all normal cellular RNA synthesis was discovere...
متن کاملRibonucleic Acid and Protein Synthesis in Mitotic Hela Cells
HeLa cells arrested in mitosis were obtained in large numbers, with only very slight interphase cell contamination, by employing the agitation method of Terasima and Tolmach, and Robbins and Marcus. Protein synthesis and RNA synthesis were almost completely suppressed in mitotic cells. Active polyribosomes were nearly absent in mitotic cells as compared with interphase cells treated in the same...
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ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1972
ISSN: 0306-3283
DOI: 10.1042/bj1290030pa